Coding

Part:BBa_K1676097:Design

Designed by: Ooi Kean Hean   Group: iGEM15_NTU-Singapore   (2015-09-18)


Mutant 1 of Lactate Permease


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 938


Design Notes

Primers were designed to have the start and stop codon so that they will not be mutated during PCR



Source

Random mutagenesis was carried out via PCR to obtain the mutants.

References